ISOGlyP's Selective Peptide


This page is used to create peptides that are specific for a given set of transferases while excluding other transferases.

General Parameters:
Glycosylation site amino acid:
Transferase T1 T2 T3 T4 T5 T10 T11 T12 T13 T14 T16
Selected-For:
Selected-Against:
Thr/Ser Ratio
Selected-For EVP Value cutoff
Selected-Against EVP Value cutoff
EV Table Version:
Positional Selection for EVP Calculations:
X X X X X T/S X X X X X
-
-5 -4 -3 -2 -1 0 +1 +2 +3 +4 +5
Sequence Submission:
Copy and paste the sequence(s) in FASTA format.
Or upload the file containing the input sequence(s) in FASTA format.
 
Or
Random Peptide Creation:
Base sequence

The base sequence allows for the creation of random peptides with variation only in locations indicated by a X. For example, AXXXNTXXXXX would have an A at -5 and N at -1, the remaining amino acids would randomly be choosen.

Amino Acids to Exclude

The amino acids to exclude allows for certain amino acids to be excluded from the creation of the random peptides. For example, to keep A or R from being used, the input would be A,R

Number of Peptides to Test

This value creates the number of peptides to randomly test. WARNING: Excessive amount of peptides can take a long time to process.

Number of Peptides to Return

This value allows for the number of top peptides that pass the filters to return.

Random number seed:

This value allows the user to repeat the analysis using the same set of random peptides. This value can either be user defined for new runs or the ID found in a prior run.

Optional: Email address to send link to results page
 

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This work was supported by Grant 5U54MD007592 from the National Institute on Minority Health and Health Disparities (NIMHD), a component of the National Institutes of Health (NIH) to UTEP's Border Biomedical Research Center for Research Resources and NIH grant NCI-CA78834 (TAG). Its contents are solely the responsibility of the authors.